白头翁汤通过保护微血管内皮细胞的完整性及PMNs迁移杀菌功能的影响

doi:10.11843/j.issn.0366-6964.2016.04.025

Abstract

Abstract:

The aims of this paper was to investigate the protective effect of pulsatillae decoction (PD) on endothelial cells injured by lipopolysaccharide (LPS) and to reveal the pharmacological of PD.The cell proliferation and cytotoxicity of the rat microvascular endothelial cells (RIMVECs) were determined by WST-1 ELISA kit and lactate dehydrogenase (LDH) assay.In addition，the RIMVECs damage stimulated by LPS was observed by hematoxylin-eosin (HE) and Hoechst 33342 staining.The transedothlial neutrophils killing was detected by gentamicin protection assay though the build of Transwell.The results showed that LPS (1 μg•mL^-1) make the nuclei of RIMVECs shriveled and damage the cell membrane of RIMVECs and lead to cell fall off.The rate of cytotoxicity was 53.2%.The rate of separated cells wass 39.6%.PD（20 μg•mL^-1）had the significant proliferative effect for that.LPS induced transendothelial migration of neutrophils can reduce the survival of bacteria in both intracellular and extracellular under the effect of 20 μg•mL^-1 PD (P＜0.01).Results reveal that PD can improve the sterilization function of PMNs by the effective protection of LPS-induced injury of RIMVECs，and might improve the sterilization function of PMNs by protecting the integrity of the RIMVECs to provide a theoretical basis for the dose of clinical treatment of bacterial animal diseases.

CLC Number:

S853.9

WANG Ming-ming，YANG Shu，DONG Hong，MU Xiang. Pulsatillae Decoction Improves the Bactericidal Capacity of Neutrophils by Protection of Integrity of Microvascular Endothelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 836-843.

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Pulsatillae Decoction Improves the Bactericidal Capacity of Neutrophils by Protection of Integrity of Microvascular Endothelial Cells

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